Igumbi lokuhlinza lobuchwepheshe obuphezulu: imakroskopu yokuhlinzwa!

 

Igumbi lokuhlinza liyindawo egcwele imfihlakalo nokwesaba, isigaba lapho izimangaliso zokuphila zenziwa khona njalo. Lapha, ukuhlanganiswa okujulile kobuchwepheshe kanye nezokwelapha akugcini nje ngokuthuthukisa izinga lempumelelo lokuhlinzwa, kodwa futhi kunikeza isithiyo esiqinile sokuphepha kwesiguli, okwenza ukwelashwa ngakunye kube yisinyathelo esiqinile sohlangothi lwezempilo. Ukusetshenziswa kabanzi kwemishini yobuchwepheshe obuphezulu kunikeze odokotela abahlinzayo amaphiko. Abagcini nje ngokuba abasizi ababalulekile etafuleni lokuhlinza, kodwa futhi “izikhali eziyimfihlo” ezivikela impilo futhi zidale izimangaliso.

Esikwethula kuwe yindoda enkulu enamashiya amakhulu namehlo amakhulu egumbini lokuhlinza: iimakroskopu yokuhlinzwaAkuyona nje kuphela umsizi othembekile kodokotela, kodwa futhi "namehlo abo egolide abukhali futhi aqondayo". Ngosizo lukaAma-microscope asebenzayo, ngisho nemithambo yegazi emincane kakhulu kanye nemizwa kungakhuliswa futhi kubonakale ngokucacile, okunikeza ukunemba okungakaze kubonwe ngaphambili kokuhlinzwa.

Ukuthatha uchungechunge lwe-CORDER ASOMimakroskopu yokuhlinzwanjengesibonelo, ukusebenza kwayo okuhle kakhulu kokubona kuqinisekisa ukucaca okuphezulu nokujula kwensimu yokuhlinza, okuvumela odokotela ukuthi bahlukanise kalula izakhiwo zezicubu futhi benze imisebenzi eyinkimbinkimbi njengokusika nokuthunga ngokunembile futhi ngaphandle kwephutha.

Ekuhlinzeni izifo zokuhlinzwa kwemizwa, ukusetshenziswaama-microscope okuhlinzwaifinyelele ekuthuthukisweni okuncane kwenqubo yokuhlinzwa, yanciphisa kakhulu umonakalo ezicutshini ezizungezile ezinempilo, yathuthukisa ukuphepha kokuhlinzwa kanye nekhwalithi yokuphila ngemva kokuhlinzwa kweziguli.

Lolu chungechunge lwe-CORDER ASOMI-microscope esebenzayoAkukhona nje kuphela ukuthi inesistimu yokukhanya ethuthukisiwe, kodwa futhi ifakwe ubuchwepheshe be-vascular fluorescence. Lobu buchwepheshe bulethe ukulula nokunemba okungakaze kubonwe enkambisweni yokuhlinzwa. Lobu buchwepheshe buhlanganisa izinkambu eziningi ze-optics, izithombe, kanye nemithi ukuze kwakhiwe uhlelo losizo lokuhlinzwa olusebenza kahle noluphephile.

Ngesikhathi sokuhlinzwa, ubuchwepheshe be-vascular fluorescence budlala indima ebalulekile. Ngokusebenzisa amamojula akhethekile okuhlukanisa i-fluorescence afana nalawaAma-microscope okuhlinzwa e-CORDER ASOM, izithombe zemithambo yegazi ezinencazelo ephezulu zinganikezwa, okuvumela odokotela ukuthi babone ngokucacile izinguquko ekugelezeni kwegazi emithanjeni yegazi futhi bathole izicubu ezigulayo ngokunembile, okusiza odokotela ukuthi bakhe izinhlelo zokuhlinzwa ezinembile kakhudlwana.

Ngosizo lobuchwepheshe be-vascular fluorescence, odokotela bangahlola nokusebenza kahle kwe-vascular anastomosis futhi babheke isakhiwo sezicubu esibhalwe nge-fluorescence ngesikhathi sangempela ngesikhathi sokuhlinzwa, okusiza ukuthola izingozi ezingaba khona zokuhlinzwa ngesikhathi esifanele futhi kuthathwe izinyathelo ezifanele.

Ngaphezu kwalokho, i-I-microscope ye-CORDER ASOMingahlukanisa phakathi kwezicubu ezivamile nezigulayo nge-module ekhethekile yokuhlukanisa i-fluorescence, inikeze ukuzulazula okunembile kwezinqubo zokuhlinzwa ezingangenisi kakhulu ezindaweni ezincane. Lokhu kwenza izinqubo zokuhlinzwa eziyinkimbinkimbi zibe lula futhi ziguquguquke kakhudlwana, okusiza ukunciphisa ukusikwa kokuhlinzwa kanye nomonakalo wezicubu.

Ngaphezu kobuchwepheshe be-vascular fluorescence, uchungechunge lwe-CORDER ASOMIsibonakhulu sokuhlinzafuthi ihlanganisa imisebenzi eminingi esezingeni eliphezulu, njengobuchwepheshe bokuthwebula izithombe obunesisombululo esiphezulu se-4K, okuletha ukucaca okungakaze kubonwe emkhakheni wokubukwa kokuhlinzwa; Ubuchwepheshe bokuzwela ukushaqeka kwerobhothi obuhlakaniphile buqinisekisa ukuzinza nokunemba ngesikhathi senqubo yokuhlinzwa; I-interface yokusebenza kwesikrini sokuthinta sedijithali ngokugcwele yenza ukulawula kokuhlinzwa kube lula futhi kube lula; Futhi ukujula okuyingqayizivele komsebenzi wokuthuthukisa insimu kuthuthukisa kakhulu ukubonakala nokunemba kokuhlinzwa. Ukuhlanganiswa kwalezi zobuchwepheshe ezithuthukisiwe ndawonye kusiza ukufeza amazinga aphezulu okunemba nokusebenza kahle enqubweni yokuhlinzwa.

Ekusebenzeni okungokoqobo, izinzuzo zochungechunge lwe-CORDER ASOMimakroskopu yokuhlinzwazibonakala nakakhulu.

Ipulatifomu yayo yokusebenza eguquguqukayo kanye nomklamo olula ukuyisebenzisa kuvumela odokotela ukuthi bavumelane nemikhuba yabo yokusebenza, okuthuthukisa kakhulu induduzo kanye nokusebenza kahle kokuhlinzwa. Ngaphezu kwalokho, i-I-microscope esebenzayoIhambisana kahle kakhulu futhi ingasetshenziswa kanye namadivayisi ahlukahlukene ezokwelapha ukuze kuhlangatshezwane nezidingo zokuhlinzwa eziyinkimbinkimbi. Ingasetshenziswa futhi kanye nokwakhiwa kwamakamelo okuhlinzwa edijithali ngaphandle kwezinkinga.